Data/hora: 07/06/2024 03:45:12

Provedor de dados: 	45	
País: 	Brazil	
Título: 	Induction of the acrosome reaction test to in vitro estimate embryo production in Nelore cattle	
Autores: 	Costa,M.Z.; Oliveira,L.Z.; Resende,M.V.; Lucio,A.C.; Perini,A.P.; Miguel,M.C.V.; Lima,V.F.M.H.	
Data: 	2010-08-01	
Ano: 	2010	
Palavras-chave: 	Cattle; Fertility; Semen; Acrosome reaction; Fertilization.	
Resumo: 	The effectiveness of induction of the acrosome reaction (AR) test as a parameter to in vitro estimate embryo production (IVP) in Nelore breed and the AR pattern by the Trypan Blue/Giemsa (TB) stain were evaluated. Frozen semen samples from ten Nelore bulls were submitted to AR induction and were also evaluated for cleavage and blastocyst rates. The treatments utilized for AR induction were: control (TALP medium), TH (TALP medium + 10μg heparin), TL (TALP medium + 100μg lysophosphatidylcholine) and THL (TALP medium + 10μg heparin + 100μg lysophosphatidylcholine). Sperm acrosomal status and viability were evaluated by TB staining at 0 and after 4h incubation at 38°C. The results obtained for AR presented a significant difference (P&lt;0.05) in the percentage of acrosome reacted live sperm after 4h of incubation in the treatments that received heparin. The cleavage and blastocyst rates were 60% and 38% respectively and a significant difference was observed among bulls (P&lt;0.05). It was founded a satisfactory model to estimate the cleavage and blastocyst rates by AR induction test. Therefore, it can be concluded that the induction of the AR test is a valuable tool to predict the IVP in Nelore breed.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400001	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/S0102-09352010000400001	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.62 n.4 2010	
Fonte: 	info:eu-repo/semantics/openAccess