Data/hora: 06/06/2024 00:41:12

Provedor de dados: 	45	
País: 	Brazil	
Título: 	Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured	
Autores: 	Lima-Verde,I.B.; Matos,M.H.T.; Bruno,J.B.; Martins,F.S.; Santos,R.R.; Báo,S.N.; Luque,M.C.A.; Vieira,G.A.B.; Silveira,E.R.; Rodrigues,A.P.R.; Figueiredo,J.R.; Oliveira,M.A.L.; Lima,P.F.	
Data: 	2009-02-01	
Ano: 	2009	
Palavras-chave: 	Caprine; Preantral follicle; Α-tocopherol; Ternatin.	
Resumo: 	The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P&lt;0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P&lt;0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.	
Tipo: 	Info:eu-repo/semantics/report	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/S0102-09352009000100009	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.61 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess