Data/hora: 01/06/2024 03:48:36

Provedor de dados: | 45
País: | Brazil
Título: | Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
Autores: | Domingues,S.F.S.; Ferreira,H.S.; Muniz,J.A.P.C.; Lima,A.K.F.; Ohashi,O.M.; Figueiredo,J.R.; Silva,L.D.M.
Data: | 2003-06-01
Ano: | 2003
Palavras-chave: | Monkey; Preantral follicle; Isolation; Ovary; Cebus apella.
Resumo: | The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352003000300009
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.55 n.3 2003
Fonte: | info:eu-repo/semantics/openAccess