Data/hora: 06/06/2024 04:38:31

Provedor de dados: 	45	
País: 	Brazil	
Título: 	Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos	
Autores: 	Pradieé,J.; Cardoso,T.F.; Silva,E.F.; Gonçalves,A.O.; Gastal,G.D.A.; Rosa,C.E.; Mondadori,R.G.; Pegoraro,L.M.C.; Vieira,A.D.; Lucia Jr.,T.	
Data: 	2016-10-01	
Ano: 	2016	
Palavras-chave: 	Sheep embryos; Antioxidant; Reactive oxygen species; Frozen ram sperm; Vitrification.	
Resumo: 	ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P&gt;0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P&gt;0.05) to those of a medium with 50μM BME and 600μM cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P&gt;0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/1678-4162-8479	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.68 n.5 2016	
Fonte: 	info:eu-repo/semantics/openAccess