Data/hora: 14/06/2024 05:21:25

Provedor de dados: | 45
País: | Brazil
Título: | In vitro evaluation of canine spermatozoa cryopreserved in different extenders
Autores: | Oliveira,E.C.S.; Juliani,G.C.; Marques Jr.,A.P.; Henry,M.
Data: | 2006-12-01
Ano: | 2006
Palavras-chave: | Dog; Semen; Cryopreservation; Ethylene glycol; Dimethyl formamide.
Resumo: | The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of post-thawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling test. Lactose-egg yolk based extenders showed better cryoprotectant capability and dimethyl formamide was an alternative cryoprotectant agent for dog sperm cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600021
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352006000600021
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.58 n.6 2006
Fonte: | info:eu-repo/semantics/openAccess