Data/hora: 06/06/2024 04:48:34

Provedor de dados: | 45
País: | Brazil
Título: | Accuracy of the estimates of ammonia concentration in rumen fluid using different analytical methods
Autores: | Souza,N.K.P.; Detmann,E.; Valadares Filho,S.C.; Costa,V.A.C.; Pina,D.S.; Gomes,D.I.; Queiroz,A.C.; Mantovani,H.C.
Data: | 2013-12-01
Ano: | 2013
Palavras-chave: | Ammonia nitrogen; Indophenol colorimetric reaction; Kjeldahl distillation.
Resumo: | The accuracy of two different methods in measuring the ammonia nitrogen (N-NH3) concentration in rumen fluid were evaluated: a catalyzed indophenol colorimetric reaction (CICR) and the Kjeldahl distillation (KD). Five buffered standard solutions containing volatile fatty acids, true protein, and known ammonia concentrations (0, 3, 6, 12, and 24 N-NH3 mg/dL) were used to simulate rumen fluid. Different ratios (10:1, 7.5:1, 5:1, 2.5:1, 1:1, 1:2.5, 1:5, 1:7.5, and 1:10) of a potassium hydroxide solution (KOH, 2 mol/L) to standard solutions were evaluated by the KD method. The accuracy of each method was evaluated by adjusting a simple linear regression model of the estimated N-NH3 concentrations on the N-NH3 concentrations in the standard solutions. When the KD method was used, N-NH3 was observed to be released from the deamination of true protein (P&lt;0.05), and an incomplete recovery of N-NH3 was observed (P&lt;0.05), except for 7.5:1 and 5:1 ratios of KOH solution to standard solutions (P&amp;gt;0.05). The estimates of the N-NH3 concentration obtained by the CICR method were found to be accurate (P&amp;gt;0.05). After the accuracy evaluation, ninety-three samples of rumen fluid were evaluated by the CICR and KD methods (using the 5:1 ratio of KOH solution to rumen fluid sample), assuming that the CICR estimates would be accurate. The N-NH3 concentrations obtained by the two methods were observed to be different (P&lt;0.05) but strongly correlated (r = 0.9701). Thus, it was concluded that the estimates obtained by the Kjeldahl distillation using a 5:1 ratio of KOH solution to rumen fluid sample can be adjusted to avoid biases. Furthermore, a model to adjust the N-NH3 concentration is suggested.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000600024
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352013000600024
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.65 n.6 2013
Fonte: | info:eu-repo/semantics/openAccess