Data/hora: 01/06/2024 01:23:58

Provedor de dados: | 45
País: | Brazil
Título: | Addition of chlorogenic acid and caffeine during the processing of cooled boar semen
Autores: | Pereira,B.A.; Rocha,L.G.P.; Teles,M.C.; Silva,W.E.; Barbosa,J.A.; Rabelo,S.S.; Uchoa,A.S.; Rodrıguez-Gil,J. E.; Pereira,L.J.; Zangeronimo,M.G.
Data: | 2019-04-01
Ano: | 2019
Palavras-chave: | Antioxidant; Boar semen storage; Metabolic activator; Methylxanthine; Polyphenol.
Resumo: | ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P &lt;0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P &lt;0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P&lt;0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P &lt;0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P &lt;0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200489
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-10415
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.71 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess