Data/hora: 06/06/2024 15:41:53

Provedor de dados: | 45
País: | Brazil
Título: | Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks
Autores: | Radulović,Ž.; Milutinović,M.; Tomanović,S.; Mulenga,A.
Data: | 2010-08-01
Ano: | 2010
Palavras-chave: | Borrelia; RT-PCR; 16S rRNA; Ixodes ricinus.
Resumo: | A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting pathogen presence, especially when in investigations is required extraction of total RNA from ticks.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352010000400015
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.62 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess