Data/hora: 10/06/2024 07:36:25

Provedor de dados: | 47
País: | Argentina
Título: | Rosette formation by macrophages with adhered T lymphocytes is precluded by inhibitors of antigen processing and presentation
Autores: | Novak,Ivón Teresa; Cabral,Humberto Ramón.
Data: | 2008-08-01
Ano: | 2008
Palavras-chave: | Autologous rosetting; Cultured human macrophages and T- lymphocytes; Brefeldin A; Chloroquine; Immune synapses.
Resumo: | We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected to either brefeldin A, chloroquine or to an anti- HLA DR antibody. Rosette formation was significantly inhibited in the treated samples (either with brefeldin A, chloroquine or the anti- HLA DR; ANOVA, p&lt;0.001, as compared with the untreated controls). It is concluded that interference with antigen processing and presentation precludes the formation of these cellcell interactions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200002
Editor: | Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
Formato: | text/html
Fonte: | Biocell  v.32 n.2 2008
Fonte: | info:eu-repo/semantics/openAccess