Data/hora: 11/06/2024 03:40:30

Provedor de dados: | 47
País: | Argentina
Título: | Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture
Autores: | Fontana,María Laura; Mroginski,Luis Amado; Rey,Hebe Yolanda.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | In vitro cultivation; Leaflet explants; Micropropagation; Thidiazuron; Wild groundnut.
Resumo: | With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: &amp;#945;-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 &amp;#956;M thidiazuron and 4.44 &amp;#956;M 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 &amp;#956;M &amp;#945;-naphthalenacetic acid, 13.95 &amp;#956;M kinetin and 13.32 &amp;#956;M 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 &amp;#956;M &amp;#945;-naphthalenacetic acid.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300006
Editor: | Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
Formato: | text/html
Fonte: | Biocell  v.33 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess