Data/hora: 01/06/2024 06:17:42

Provedor de dados: 	47	
País: 	Argentina	
Título: 	Endogenous ADP-ribosylation of eukaryotic elongation factor 2 and its 32 kDa tryptic fragment	
Autores: 	Ergen,,Kivanç; Bektas¸,Muhammet; Gökçe,Sina; Nurten,Rüstem.	
Data: 	2007-04-01	
Ano: 	2007	
Palavras-chave: 	Eukaryotic elongation factor 2; Endogenous ADP-ribosylation; Protein synthesis; Trypsin digestion; Diphtheria toxin.	
Resumo: 	Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis. In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related to protein synthesis. This modification appears to be important for the cell function.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100007	
Editor: 	Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)	
Formato: 	text/html	
Fonte: 	Biocell  v.31 n.1 2007	
Fonte: 	info:eu-repo/semantics/openAccess