Data/hora: 10/06/2024 05:40:35

Provedor de dados: | 47
País: | Argentina
Título: | Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
Autores: | Lopez,Lidia M.; Dubin,Marta; Carrizo,Patricia H.; Burgos,Mario H.; Pellegrino de Iraldi,Amanda; Stoppani,Andrés O. M.
Data: | 2003-08-01
Ano: | 2003
Palavras-chave: | Apoptosis; O-naphthoquinones; Hepatocytes; Chromatin Alteration; Mitochondrial Swelling Membrane; Blebs.
Resumo: | CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho&amp;#091;1,2b&amp;#093;pyran- 5,6-dione; CG-NQ), a b-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen peroxide. Expression of such effects varied according to hepatocyte samples and taken together strongly support an apoptotic action of CG-NQ dependent on "reactive oxygen species"
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200007
Editor: | Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
Formato: | text/html
Fonte: | Biocell  v.27 n.2 2003
Fonte: | info:eu-repo/semantics/openAccess