Data/hora: 13/06/2024 20:38:43

Provedor de dados: | 48
País: | Chile
Título: | Zinc in the prevention of Fe2initiated lipid and protein oxidation
Autores: | ZAGO,M. PAOLA; VERSTRAETEN,SANDRA V.; OTEIZA,PATRICIA I.
Data: | 2000-01-01
Ano: | 2000
Palavras-chave: | Aluminum; Catechins; Free radicals; Iron; Lipid peroxidation; Zinc.
Resumo: | In the present study we characterized the capacity of zinc to protect lipids and proteins from Fe2+-initiated oxidative damage. The effects of zinc on lipid oxidation were investigated in liposomes composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS) at a molar relationship of 60:40 (PC:PS, 60:40). Lipid oxidation was evaluated as the oxidation of cis-parinaric acid or as the formation of 2-thiobarbituric acid-reactive substances (TBARS). Zinc protected liposomes from Fe2+ (2.5-50 muM)-supported lipid oxidation. However, zinc (50 muM) did not prevent the oxidative inactivation of glutamine synthelase and glucose 6-phosphate dehydrogenase when rat brain superntants were oxidized in the presence of 5 muM Fe2+ and 0.5 mM H2O2 .We also studied the interactions of zinc with epicatechin in the prevention of liid oxidation in liposomes. The simulaneous addition of 0.5 muM epicatechin (EC) and 50 muM zinc or EC separately. Zinc (50 muM) also protecte liposomes from the stimulatory effect of aluminum on Fe2+-initiated lipid oxidation. Zinc could play an important role as an antioxidant in biological systems, replacing iron and other metals with pro-oxidant activity from binding sites and interacting with other components of the oxidant defense system.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200014
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.33 n.2 2000