Data/hora: 01/06/2024 07:22:56

Provedor de dados: | 48
País: | Chile
Título: | A simple and effective pressure culture system modified from a transwell cell culture system
Autores: | Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian.
Data: | 2013-01-01
Ano: | 2013
Palavras-chave: | Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton.
Resumo: | Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin &amp;#946;1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.46 n.1 2013