Data/hora: 01/06/2024 15:31:13

Provedor de dados: | 48
País: | Chile
Título: | Expression of non-neuronal cholinergic system in maxilla of rat in vivo
Autores: | Guo,Jie; Wang,Lue; Xu,Haihua; Che,Xiaoxia.
Data: | 2014-01-01
Ano: | 2014
Palavras-chave: | Non-neuronal cholinergic system; Maxilla; Real-Time PCR; Immunohistochemistry.
Resumo: | BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits &amp;#945;1, &amp;#945;2, &amp;#945;3, &amp;#945;5, &amp;#945;7, &amp;#945;10, &amp;#946;1, &amp;#946;2, &amp;#946;4, &amp;#947; and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits &amp;#945;2, &amp;#946;1, &amp;#946;4 and mAChR subunits M4 had abundant expression (2-&amp;#916;Ct&amp;#8201;&gt;&amp;#8201;0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChR&amp;#945;7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor &amp;#945;7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells. CONCLUSIONS: ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.47 2014