Data/hora: 05/06/2024 08:12:28

Provedor de dados: | 48
País: | Chile
Título: | Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
Autores: | JAIMOVICH,ENRIQUE; ESPINOSA,ALEJANDRA.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Skeletal; Transcription factors; Insulin; IGF-I; Testosterone.
Resumo: | We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (&lt;1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The different intracellular Ca2+ patterns produced by electrical stimulation, testosterone, IGF-I and insulin, may help to understand the role of intracellular calcium kinetics in the regulation of gene expression by various stimuli in skeletal muscle cells.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.37 n.4 2004