Data/hora: 15/06/2024 03:06:05

Provedor de dados: | 48
País: | Chile
Título: | Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
Autores: | MISHRA,PANKAJ K; MISHRA,SMITA; BISHT,SHEKHAR C; SELVAKUMAR,G; KUNDU,S; BISHT,J. K; GUPTA,HARI SHANKAR.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Cold tolerant; Pseudomonas lurida; 16S rRNA gene; Nutrient uptake; Plant growth promotion.
Resumo: | A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 &amp;#956;m in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6% (wt/vol). Although it was sensitive to Rifampicin (R 20 &amp;#956;g mi-1), Gentamicin (G 3 &amp;#956;g mi-1), and Streptomycin (S 5 &amp;#956;g mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 &amp;#956;g mi-1), Penicillin (P 300 &amp;#956;g mi-1), Polymixin B sulphate (Pb 100 &amp;#956;g mi-1) and Chloramphenicol (C 200 &amp;#956;g mi-1). The 16S rRNA sequence analysis revealed maximum identity with Pseudomonas lurida. The bacterium produced indole Acetic Acid (IAA) and solubilizes phosphate at 4, 15 and 28°C. It also retained its ability to produce rhamnolipids and siderophores at 15°C. Seed bacterization with the isolate enhanced the germination, shoot and root lengths of thirty-day-old wheat seedlings by 19.2, 30.0 &amp;amp; 22.9% respectively, as compared to the un-inoculated controls.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.42 n.3 2009