Data/hora: 05/06/2024 13:23:59

Provedor de dados: | 48
País: | Chile
Título: | Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
Autores: | AVILA,ANA D; CALDERÓN,CARLOS F; PEREZ,RITA M; PONS,CARMEN; PEREDA,CELIA M; ORTIZ,ANA R.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Epidermal growth factor; Epidermal growth factor receptor; Hemolytic toxins; Immunotoxins; Monoclonal antibodies.
Resumo: | Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptor
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.40 n.2 2007