Data/hora: 06/06/2024 03:36:36

Provedor de dados: 	48	
País: 	Chile	
Título: 	Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers	
Autores: 	VERGARA,JULIO L; DIFRANCO,MARINO.	
Data: 	2006-01-01	
Ano: 	2006	
Palavras-chave: 	Excitation-contraction coupling; Caffeine; Confocal spot detection; Calcium transients; Frog skeletal muscle; Calcium microdomains.	
Resumo: 	The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines where the triads are located and secondary actions on other potential Ca Release sources. Our results demonstrate that 0.5 mM caffeine potentiates and prolongs localized action-potential evoked Ca2+ transients recorded at the level of the Z-lines, but that 1mM only prolongs them. The effects at both doses are reversible. At the level of the M-line, localized Ca2+ transients displayed more variability in the presence of 1 mM caffeine than in control conditions. At this dose of caffeine, extra-junctional sources of Ca2+ release also were observed occasionally.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300017	
Editor: 	Sociedad de Biología de Chile	
Formato: 	text/html	
Fonte: 	Biological Research  v.39 n.3 2006