Data/hora: 07/06/2024 08:54:56

Provedor de dados: | 48
País: | Chile
Título: | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
Autores: | Almeida,Fabiana Greyce Oliveira; Aquino,Priscila Ferreira de; Souza,Afonso Duarte Leäo de; Souza,Antonia Queiroz Lima de; Vinhote,Sonia do Carmo; Mac-Cormick,Thais Messias; Silva,Marcelo Soares da Mota; Chalub,Sidney Raimundo Silva; Fischer,Juliana de Saldanha da Gama; Carvalho,Paulo Costa; Carvalho,Maria da Gloria da Costa.
Data: | 2015-01-01
Ano: | 2015
Palavras-chave: | Methylation; Colorectal cancer; Amazon; Epigenetic.
Resumo: | BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. CONCLUSIONS: Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.48 2015