Data/hora: 07/06/2024 02:20:33

Provedor de dados: | 5
País: | France
Título: | The dispersion of adhered marine bacteria by pyrophosphate and ultrasound prior to direct counting.
Autores: | Velji, M; Albright, L.
Data: | 1984-10
Ano: | 1984
Palavras-chave: | Bacteria; Microbiological analysis; Flocculation; Ultrasonics; Bacterial counters.
Resumo: | A technique has been investigated for dispersing adhered marine bacteria from various surfaces prior to direct counting. The bacterial cells were initially preserved and strengthened by use of 3.7% formaldehyde (v/v final concentration). Seawater samples were then treated with 0.001 M of tetrasodium pyrophosphate (final concentration), a sequestering and deflocculating agent. Surface sediment and kelp disc samples were treated with 0.01 M tetrasodium pyrophosphate (final concentration) after having been suspended in 0.44 M sodium chloride solution. These samples were incubated for 15-30 min. Subsequently, the samples were treated with ultrasound (100 W) for 30 to 45 s. This technique resulted in an even dispersion of most bacterial cells in the samples. Bacterial cells dispersed in this manner were then stained with 4',6-diamino-2-phenylindole (DAPI) and enumerated by standard epifluorescent microscopy. Bacterial populations of surface sediment and older kelp blades which were previously difficult or impossible to enumerate were now found to be easily determined. A mechanism is proposed to explain the increase in dispersion of the bacterial cells by this technique.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/1984/acte-968.pdf
Editor: | Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986
Relação: | http://archimer.ifremer.fr/doc/00000/968/
Formato: | application/pdf