Data/hora: 06/06/2024 12:16:19

Provedor de dados: 	5	
País: 	France	
Título: 	A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen	
Autores: 	Mesiet Cladiere, L; Norais, Cédric; Kuhn, Joelle; Briffotaux, Julien; Sloostra, Jerry W.; Ferrari, Elena; Hubscher, Ulrich; Flament, Didier; Myllykallio, Hannu.	
Data: 	2007-10	
Ano: 	2007	
Palavras-chave: 	Ribonuclease HII; Combinatorial peptide synthesis; PCNA.	
Resumo: 	During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human proteins.   (c) 2007 Elsevier Ltd. All rights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2007/publication-3297.pdf

DOI:10.1016/j.jmb.2007.06.056	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/3297/	
Formato: 	application/pdf	
Fonte: 	Journal of Molecular Biology (0022-2836) (Elsevier), 2007-10 , Vol. 372 , N. 5 , P. 1137-1148	
Fonte: 	2007 Elsevier Ltd All rights reserved