Data/hora: 06/06/2024 11:02:34

Provedor de dados: | 5
País: | France
Título: | Recherche et caractérisation d'immunoglobulines dans les oeufs du loup (Dicentrarchus labrax)
Autores: | Pepin, Jean-francois; Breuil, Gilles; Rigobert, P; Romestand, Bernard.
Data: | 1997
Ano: | 1997
Palavras-chave: | Immunoglobuline; Transfert immunité maternelle; ELISA; Œuf; Poisson; Immunoglobulin; Maternal immunity transfer; ELISA; Eggs; Fish.
Resumo: | The occurrence of maternal immunity transfer in Sea bass (Dicentrarchus labrax) was investigated using ELISA and Western blot technics. In order to determine the possible presence of immunoglobulin (Ig), egg proteins obtained from fertilized eggs were analyzed. After several extraction protocol assays (precipitation, chromatography), monoclonal and polyclonal antibody probes specific to Sea bass Ig allowed to confirm the presence of an immunoglobulin-like protein in eggs with very low level. Biochemical characterization was carried out using SDS-PAGE. Results indicated that egg Ig had a molecular weight of 206 kDa. They were smaller than seric IgM (800 kDa). The heavy (H) and light (L) chains of the egg Ig and of the seric IgM had similar molecular weight, 75 kDa and 28 kDa, respectively. These data suggest that egg Ig of Sea bass have monomeric structure. We hypothesize they could be sub-unit from the tetrameric IgM
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00272/38275/36458.pdf

http://archimer.ifremer.fr/doc/00272/38275/

Editor: | Société d'ichtyophysiologie fondamentale et appliquée
Formato: | application/pdf
Fonte: | Ichtyophysiologica Acta (0245-8608) (Société d'ichtyophysiologie fondamentale et appliquée), 1997 , Vol. 20 , P. 77-85

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