Data/hora: 07/06/2024 02:20:29

Provedor de dados: | 5
País: | France
Título: | An improved whole life cycle culture protocol for the hydrozoan genetic model   Clytia hemisphaerica
Autores: | Lechable, Marion; Jan, Anne-cecile; Duchene, Axel; Uveira, Julie; Weissbourd, Brandon; Gissat, Loann; Collet, Sophie; Gilletta, Laurent; Chevalier, Sandra; Leclère, Lucas; Peron, Sophie; Barreau, Carine; Lasbleiz, Régis; Houliston, Evelyn; Momose, Tsuyoshi.
Data: | 2020-11
Ano: | 2020
Palavras-chave: | Animal culture; Cnidarian; Developmental biology; Genetics; Jellyfish.
Resumo: | The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00656/76820/77996.pdf

https://archimer.ifremer.fr/doc/00656/76820/77997.pdf

DOI:10.1242/bio.051268

https://archimer.ifremer.fr/doc/00656/76820/

Editor: | The Company of Biologists
Formato: | application/pdf
Fonte: | Biology Open (2046-6390) (The Company of Biologists), 2020-11 , Vol. 9 , N. 11 , P. bio051268 (13p.)

Fonte:

info:eu-repo/semantics/openAccess

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