Data/hora: 01/06/2024 03:25:38

Provedor de dados: | 5
País: | France
Título: | Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei
Autores: | Labreuche, Yannick; Veloso, Artur; De La Vega, Enrique; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.
Data: | 2010-11
Ano: | 2010
Palavras-chave: | Shrimp; Litopenaeus vannamei; RNAi; DsRNA; Argonaute; Sid-1; Antiviral immunity.
Resumo: | Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both RNAi-related and innate antiviral responses. They also suggest that these two mechanisms of antiviral response may activate the same pathway, requiring Lv-Sid 1 and Lv-Ago 2 induction. (C) 2010 Elsevier Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00015/12652/9587.pdf

DOI:10.1016/j.dci.2010.06.017

Editor: | Elsevier Sci Ltd
Relação: | http://archimer.ifremer.fr/doc/00015/12652/
Formato: | application/pdf
Fonte: | Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2010-11 , Vol. 34 , N. 11 , P. 1209-1218
Fonte: | 2010 Elsevier Ltd All rights reserved.