Data/hora: 11/06/2024 06:41:11

Provedor de dados: | 5
País: | France
Título: | Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement
Autores: | Geiger, Marie.
Data: | 2013-09-25
Ano: | 2013
Palavras-chave: | Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.
Resumo: | The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three types of assays: cytotoxicity on KB cells, a bioassay on diptera larvae and antibacterial activity on marine bacteria. These bioassays were first adapted to routine protocols, subsequently their detection perimeter was evaluated at different levels of matrix complexity: known or emerging pure toxin, crude extract of microorganisms producing the toxin, and spiked shellfish matrices. These three bioassays exhibited a complementary scope of detection, and an approach for their implementation was proposed.
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00183/29426/27787.pdf

http://archimer.ifremer.fr/doc/00183/29426/

Editor: | Université de Nantes. Facultés des Sciences Pharmaceutiques.Ecole doctorale végétal, environnement, nutrition, agroalimentaire, mer
Formato: | application/pdf

Fonte:

2013 Université de Nantes

info:eu-repo/semantics/openAccess

restricted use