Data/hora: 07/06/2024 09:39:25

Provedor de dados: | 5
País: | France
Título: | Toward Detection of Harmful Algae Blooms by in situ Surface Plasmon Resonance Spectroscopy
Autores: | Laurent, Sebastien; Colas, Florent; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Lehaitre, Michel; Compere, Chantal.
Data: | 2009-02
Ano: | 2009
Palavras-chave: | PCR; Algal detection; In situ; Spectroscopy; Surface plasmon resonance.
Resumo: | Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2009/publication-6830.pdf

DOI:10.1109/SAS.2009.4801771

Editor: | IEEE
Relação: | http://archimer.ifremer.fr/doc/00000/6830/
Formato: | application/pdf
Fonte: | Sensors Applications Symposium, 2009. SAS 2009. IEEE (IEEE), 2009-02 , P. 29-33
Fonte: | 2009 IEEE – All Rights Reserved