Data/hora: 17/06/2024 06:55:44

Provedor de dados: | 5
País: | France
Título: | Microbial extracellular enzyme detection on agar plates by means of fluorogenic methylum-belliferyl-substrates.
Autores: | Kim, S; Hoppe, H.
Data: | 1984-10
Ano: | 1984
Palavras-chave: | Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques.
Resumo: | A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/1984/acte-959.pdf
Editor: | Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986
Relação: | http://archimer.ifremer.fr/doc/00000/959/
Formato: | application/pdf