Data/hora: 08/06/2024 19:17:27

Provedor de dados: 	5	
País: 	France	
Título: 	Microbial extracellular enzyme detection on agar plates by means of fluorogenic methylum-belliferyl-substrates.	
Autores: 	Kim, S; Hoppe, H.	
Data: 	1984-10	
Ano: 	1984	
Palavras-chave: 	Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques.	
Resumo: 	A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/1984/acte-959.pdf	
Editor: 	Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986	
Relação: 	http://archimer.ifremer.fr/doc/00000/959/	
Formato: 	application/pdf