Data/hora: 09/06/2024 12:29:07

Provedor de dados: 	5	
País: 	France	
Título: 	Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas	
Autores: 	Barranger, Audrey; Heude-berthelin, Clothilde; Rouxel, Julien; Adeline, Beatrice; Benabdelmouna, Abdellah; Burgeot, Thierry; Akcha, Farida.	
Data: 	2016-02	
Ano: 	2016	
Palavras-chave: 	Crassostrea gigas; Oxidative stress; DNA damage; Germ cell; Pesticides.	
Resumo: 	Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00295/40655/39753.pdf

DOI:10.1016/j.cbpc.2015.11.002

http://archimer.ifremer.fr/doc/00295/40655/	
Editor: 	Elsevier Science Inc	
Formato: 	application/pdf	
Fonte: 	Comparative Biochemistry And Physiology C-toxicology &amp; Pharmacology (1532-0456) (Elsevier Science Inc), 2016-02 , Vol. 180 , P. 23-30	
Fonte: 	2015 Elsevier Inc. All rights reserved

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