Data/hora: 07/06/2024 08:07:16

Provedor de dados: | 5
País: | France
Título: | Characterization of a gonad-specific transforming growth factor-beta superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas
Autores: | Fleury, Elodie; Fabioux, Caroline; Lelong, C; Favrel, P; Huvet, Arnaud.
Data: | 2008-02
Ano: | 2008
Palavras-chave: | Reproduction; Oyster; Gene expression; Transforming growth factor; Bivalve mollusc.
Resumo: | Through differential screening between oyster families selected for high and low summer survival, we have characterized a new transforming growth factor-beta (TGF-beta) superfamily member. This novel factor, named oyster-gonadal-TGF beta-like (og-TGF beta-like), is synthesized as a 307 amino acid precursor and displays 6 of the 7 characteristic cysteine residues of the C-terminal, mature peptide. Sequence comparison revealed that og-TGF beta-like has a low percentage of identity with other known TGF-beta superfamily members, suggesting that og-TGF beta-like is a derived member of this large superfamily. Real-time PCR (RT-PCR) analysis in different oyster tissues showed that og-TGF beta-like is specifically expressed in both male and female gonads, at distinct levels according to the reproductive stage. Og-TGF beta-like relative expression was the lowest at the initiation of the reproductive cycle and increased as maturation proceeded to achieve a maximal level in fully mature female and male oysters. In situ hybridisation demonstrated that expression was exclusively detected in the somatic cells surrounding oocytes and spermatocytes. The role of this newly-characterized TGF beta member in the reproduction of cupped oyster is discussed in regard to the specificity and the localization of its expression, which singularly contrasts with the pleiotropic roles in a variety of physiological processes commonly ascribed to most TGF-beta family members identified so far. (C) 2007 Elsevier B.V. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2008/publication-3899.pdf

DOI:10.1016/j.gene.2007.12.017

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/3899/
Formato: | application/pdf
Fonte: | Gene (0378-1119) (Elsevier), 2008-02 , Vol. 410 , N. 1 , P. 187-196
Fonte: | 2008 Elsevier B.V. All rights reserved.