Data/hora: 07/06/2024 01:32:55

Provedor de dados: 	5	
País: 	France	
Título: 	Genetic diversity and population structure of Tenacibaculum maritimum, a serious bacterial pathogen of marine fish: from genome comparisons to high throughput MALDI-TOF typing	
Autores: 	Bridel, Sébastien; Bourgeon, Frédéric; Marie, Arnaud; Saulnier, Denis; Pasek, Sophie; Nicolas, Pierre; Bernardet, Jean-françois; Duchaud, Eric.	
Data: 	2020-05	
Ano: 	2020	
Resumo: 	Tenacibaculum maritimum is responsible for tenacibaculosis, a devastating marine fish disease. This filamentous bacterium displays a very broad host range and a worldwide geographical distribution. We analyzed and compared the genomes of 25 T. maritimum strains, including 22 newly draft-sequenced genomes from isolates selected based on available MLST data, geographical origin and host fish. The genome size (~3.356 Mb in average) of all strains is very similar. The core genome is composed of 2116 protein-coding genes accounting for ~75% of the genes in each genome. These conserved regions harbor a moderate level of nucleotide diversity (~0.0071 bp−1) whose analysis reveals an important contribution of recombination (r/m ≥ 7) in the evolutionary process of this cohesive species that appears subdivided into several subgroups. Association trends between these subgroups and specific geographical origin or ecological niche remains to be clarified. We also evaluated the potential of MALDI-TOF-MS to assess the variability between T. maritimum isolates. Using genome sequence data, several detected mass peaks were assigned to ribosomal proteins. Additionally, variations corresponding to single or multiple amino acid changes in several ribosomal proteins explaining the detected mass shifts were identified. By combining nine polymorphic biomarker ions, we identified combinations referred to as MALDI-Types (MTs). By investigating 131 bacterial isolates retrieved from a variety of isolation sources, we identified twenty MALDI-Types as well as four MALDI-Groups (MGs). We propose this MALDI-TOF-MS Multi Peak Shift Typing scheme as a cheap, fast and an accurate method for screening T. maritimum isolates for large-scale epidemiological surveys.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00630/74174/73747.pdf

https://archimer.ifremer.fr/doc/00630/74174/73748.pdf

https://archimer.ifremer.fr/doc/00630/74174/73749.pdf

https://archimer.ifremer.fr/doc/00630/74174/73750.pdf

https://archimer.ifremer.fr/doc/00630/74174/73751.pdf

https://archimer.ifremer.fr/doc/00630/74174/73752.pdf

https://archimer.ifremer.fr/doc/00630/74174/73753.pdf

https://archimer.ifremer.fr/doc/00630/74174/73754.pdf

https://archimer.ifremer.fr/doc/00630/74174/73755.pdf

https://archimer.ifremer.fr/doc/00630/74174/73756.pdf

DOI:10.1186/s13567-020-00782-0

https://archimer.ifremer.fr/doc/00630/74174/	
Editor: 	Springer Science and Business Media LLC	
Formato: 	application/pdf	
Fonte: 	Veterinary Research (0928-4249) (Springer Science and Business Media LLC), 2020-05 , Vol. 51 , N. 1 , P. 60 (17p.)	
Fonte: 	info:eu-repo/semantics/openAccess

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