Data/hora: 09/06/2024 13:36:49

Provedor de dados: | 5
País: | France
Título: | Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria
Autores: | Ortega Morales, Benjamín Otto; Santiago Garcia, J.l; Chan Bacab, M.j.; Moppert, Xavier; Miranda Tello, E; Fardeau, M.l.; Carrero, J.c.; Bartolo Perez, P.; Valadez Gonzalez, A; Guezennec, Jean.
Data: | 2007
Ano: | 2007
Palavras-chave: | Tropical intertidal biofilm bacteria; Surfactants; Microbacterium sp; Exopolymers; Bacillus sp; Anionic.
Resumo: | Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS).   Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis.   Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B-22 suggests its potential biomedical application in tissue regeneration.   Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-2530.pdf

DOI:10.1111/j.1365-2672.2006.03085.x

Editor: | Blackwell science
Relação: | http://archimer.ifremer.fr/doc/00000/2530/
Formato: | application/pdf
Fonte: | Journal of Applied Microbiology (1364-5072) (Blackwell science), 2007 , Vol. 102 , N. 1 , P. 254-264
Fonte: | 2006 Blackwell