Data/hora: 01/06/2024 06:38:52

Provedor de dados: | 5
País: | France
Título: | Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community
Autores: | Durand, Lucile; Zbinden, Magali; Cueff-gauchard, Valerie; Duperron, Sebastien; Roussel, Erwan; Shillito, Bruce; Cambon-bonavita, Marie-anne.
Data: | 2010-02
Ano: | 2010
Palavras-chave: | Deferribacteres; Midgut epibiosis; Mollicutes; Proteobacteria; Rimicaris exoculata; Starvation experiment.
Resumo: | Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow and Trans-Atlantic Geotraverse sites in order to expel the transient gut contents. Microbial communities associated with the gut of starved and reference shrimps were compared using 16S rRNA gene libraries and microscopic observations (light, transmission and scanning electron microscopy and FISH analyses). We show that the gut microbiota of shrimps from both sites included mainly Deferribacteres, Mollicutes, Epsilon- and Gammaproteobacteria. For the first time, we have observed filamentous bacteria, inserted between microvilli of gut epithelial cells. They remained after starvation periods in empty guts, suggesting the occurrence of a resident microbial community. The bacterial community composition was the same regardless of the site, except for Gammaproteobacteria retrieved only in Rainbow specimens. We observed a shift in the composition of the microbiota of long-starved specimens, from the dominance of Deferribacteres to the dominance of Gammaproteobacteria. These results reinforce the hypothesis of a symbiotic relationship between R. exoculata and its gut epibionts.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00000/11142/7919.pdf

DOI:10.1111/j.1574-6941.2009.00806.x

Editor: | Wiley-blackwell Publishing, Inc
Relação: | http://archimer.ifremer.fr/doc/00000/11142/
Formato: | application/pdf
Fonte: | Fems Microbiology Ecology (0168-6496) (Wiley-blackwell Publishing, Inc), 2010-02 , Vol. 71 , N. 2 , P. 291-303
Fonte: | 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved