Data/hora: 06/06/2024 19:08:22

Provedor de dados: | 5
País: | France
Título: | Binding to PCNA in Euryarchaeal DNA Replication Requires Two PIP Motifs for DNA Polymerase D and One PIP Motif for DNA Polymerase B
Autores: | Castrec, Benoit; Rouillon, Christophe; Henneke, Ghislaine; Flament, Didier; Querellou, Joel; Raffin, Jean-paul.
Data: | 2009-11
Ano: | 2009
Palavras-chave: | Archaea; PIP box; PCNA binding motifs; DNA polymerases; DNA replication.
Resumo: | Replicative DNA polymerases possess a canonical C-terminal proliferating cell nuclear antigen (PCNA)-binding motif termed the PCNA-interacting protein (PIP) box. We investigated the role of the PIP box on the functional interactions of the two DNA polymerases, PabPol B (family B) and PabPol D (family D), from the hyperthermophilic euryarchaeon Pyrococcus abyssi, with its cognate PCNA. The PIP box was essential for interactions of PabPol B with PCNA, as shown by surface plasmon resonance and primer extension studies. In contrast, binding of PabPol D to PCNA was affected only partially by removing the PIP motif. We identified a second palindromic PIP box motif at the N-terminus of the large subunit of PabPol D that was required for the interactions of PabPol D with PCNA. Thus, two PIP motifs were needed for PabPol D for binding to PabPCNA. Moreover, the C-terminus of PabPCNA was essential for stimulation of PabPol D activity but not for stimulation of PabPol B activity. Neither DNA polymerase interacted with the PabPCNA interdomain connecting loop. Our data suggest that distinct processes are involved in PabPol D and PabPol B binding to PCNA, raising the possibility that Archaea require two mechanisms for recruiting replicative DNA polymerases at the replication fork. (C) 2009 Elsevier Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2009/publication-7317.pdf

DOI:10.1016/j.jmb.2009.09.044

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/7317/
Formato: | application/pdf
Fonte: | Journal of Molecular Biology (0022-2836) (Elsevier), 2009-11 , Vol. 394 , N. 2 , P. 209-218
Fonte: | 2009 Elsevier B.V. All rights reserved.