Data/hora: 11/06/2024 08:57:11

Provedor de dados: | 5
País: | France
Título: | Etude de nouvelles cibles moleculaires de cancer bronchopulmonaire non a petites cellules pharmacomodulées par des substances originales naturelles et synthetiques
Autores: | Moreau, Dimitri.
Data: | 2006-11-23
Ano: | 2006
Palavras-chave: | Microalgae; Natural products; Apoptosis; Antiproliferativ; Gene expression; Regulatory RNA; Molecular targets; Bronchopulmonary cancer; Microalgues; Molécules naturelles; Apoptose; Antiprolifératif; Expression génique; ARN régulateur; Cibles moléculaires; Cancer bronchopulmonaire.
Resumo: | Still rare disease at the beginning of the 20th century, the lung cancer is at the origin of more than 6 % of the deaths in France currently. It occupies there the first rank of the deaths by cancer whereas France is regarded as a country at the average risk among the Western countries. The discovery of new anti-cancer treatments increasingly more effective and specific, is thus today of primary importance.  The validation of the capacity of a molecule to being able to inhibit the cancerous proliferation, passes by several stages of tests starting with the description of an activity at the cellular level. One of the strategies used for the discovery of new compounds antiproliferativs, passes by an investigation of the bioactiv potential of a great number of chemical compounds (the screening) on cancerous cellular lines, here NSCLC-N6 and A549. These compounds can have various origins from the natural environment to the chemistry of synthesis. The studied molecules are here:  - Extracts of origin marinates produced by microalgues. 10 different species resulting from 7 phylogenetic classes were studied. The results of the first tests of activity made it possible to select several extracts to undertake a thorough chemical study aiming to isolate and identify the active ingredients (Moreau et al., 2006).  - A triazine under protection resulting from the chemistry of synthesis (A190).  As we said the specificity of the new treatments is a priority, this is why research undertaken here aims at describing the genomic mechanisms implied in the stop of the proliferation of the cancerous cells. The description of the action of a molecule on the genomic level implies the study of the transcriptome and/or the proteome. Thus during various laboratory works two particular targets have been identified by RF-Differential display: gene HEF1 and the ARN not translated B2 coded by an intronic area of HEF1. During this thesis we followed the implication of the A190 molecule in the modulation of these genes and we have tried to understand their implications in the cellular cycle (Moreau et al., 2006 (submitted)). The potential antitumor of the A190 molecule finally was studied during an in vivo experimentation and appeared very convincing.
Tipo: | Text
Idioma: | Francês
Identificador: | http://archimer.ifremer.fr/doc/2006/these-2196.pdf
Editor: | Université de Nantes
Relação: | http://archimer.ifremer.fr/doc/00000/2196/
Formato: | application/pdf