Data/hora: 06/06/2024 08:01:03

Provedor de dados: 	5	
País: 	France	
Título: 	First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway	
Autores: 	Montagnani, Caroline; Avarre, Jean-christophe; De Lorgeril, Julien; Quiquand, M; Boulo, Viviane; Escoubas, Jean-michel.	
Data: 	2007	
Ano: 	2007	
Palavras-chave: 	Damage associated molecular pattern; Tissue inhibitor of metalloproteinase; Metalloproteinase; Mollusk immunity; Crassostrea gigas; Oyster.	
Resumo: 	In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappa B binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed. (c) 2006 Elsevier Ltd. All rights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2007/publication-2094.pdf

DOI:10.1016/j.dci.2006.04.002	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/2094/	
Formato: 	application/pdf	
Fonte: 	Developmental &amp; Comparative Immunology (0145-305X) (Elsevier), 2007 , Vol. 31 , N. 1 , P. 1-11	
Fonte: 	2006 Elsevier