Data/hora: 06/06/2024 06:28:42

Provedor de dados: 	5	
País: 	France	
Título: 	Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels	
Autores: 	Li, Erchao; Arena, Leticia; Lizama, Gabriel; Gaxiola, Gabriela; Cuzon, Gerard; Rosas, Carlos; Chen, Liqiao; Van Wormhoudt, Alain.	
Data: 	2011-03	
Ano: 	2011	
Palavras-chave: 	Litopenaeus vannamei; Gene expression; Glutamate dehydrogenase; Na+-K+ ATPase; Protein; Salinity.	
Resumo: 	Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance, glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression,, and GDH activity in muscles and gills. Three experimental diets were formulated, containing 25%, 40%, and 50% dietary protein, and fed to the shrimp at a salinity of 25. After 20 days, no significant difference was observed in weight gain, though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently, shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5, respectively, and sampled at weeks 1 and 2. Shrimp fed with 40% protein at 25 in salinity (optimal conditions) were used as a control. Regardless of the salinities, shrimp fed with 50% dietary protein had significantly higher growth performance than other diets; no significant differences were found in comparison with the control. Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks. Ambient salinity change also stimulated the hepatosomatic index, which increased in the first week and then recovered to a relatively normal level, as in the control, after 2 weeks. These findings indicate that in white shrimp, the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism. Increased dietary protein level could improve the osmoreaulation capacity of L. vannamei with more energy resources allocated to GDH activity and expression.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00031/14261/11692.pdf

DOI:10.1007/s00343-011-0093-8	
Editor: 	Science Press	
Relação: 	http://archimer.ifremer.fr/doc/00031/14261/	
Formato: 	application/pdf	
Fonte: 	Chinese Journal Of Oceanology And Limnology (0254-4059) (Science Press), 2011-03 , Vol. 29 , N. 2 , P. 343-349	
Fonte: 	Chinese Scoiety for Oceanology and Limnology, Science Press, Springer-Verlag Berlin 2011