Data/hora: 01/06/2024 05:26:40

Provedor de dados: 	5	
País: 	France	
Título: 	A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas	
Autores: 	Corporeau, Charlotte; Groisillier, Agnes; Jeudy, Alexandra; Barbeyron, Tristan; Fleury, Elodie; Fabioux, Caroline; Czjzek, Mirjam; Huvet, Arnaud.	
Data: 	2011-10	
Ano: 	2011	
Palavras-chave: 	Transforming growth factor-beta; Bacterial expression; In vivo antibody inhibition; Reproduction; Crassostrea gigas; Marine bivalve.	
Resumo: 	The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00042/15371/13438.pdf

DOI:10.1007/s10126-010-9361-4	
Editor: 	Springer	
Relação: 	http://archimer.ifremer.fr/doc/00042/15371/	
Formato: 	application/pdf	
Fonte: 	Marine Biotechnology (1436-2228) (Springer), 2011-10 , Vol. 13 , N. 5 , P. 971-980	
Fonte: 	Springer Science+Business Media, LLC 2011