Data/hora: 01/06/2024 02:05:43

Provedor de dados: 	5	
País: 	France	
Título: 	Changes in biochemical and hemocyte parameters of the pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid	
Autores: 	Delaporte, Maryse; Chu, Fu-lin; Langdon, Chris; Moal, Jeanne; Lambert, Christophe; Samain, Jean-francois; Soudant, Philippe.	
Data: 	2007-05	
Ano: 	2007	
Palavras-chave: 	Polyunsaturated fatty acids; Immunology; Hemocytes; Eicosapentaenoic acid 20 : 5n 3; Crassostrea gigas; Bivalves.	
Resumo: 	The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50% and, to a lesser extent, 10% lipid emulsions had a higher total lipid content compared with oysters fed other diets, suggesting a more advanced reproductive status for the oysters fed high doses of lipid emulsion. Moreover, some oysters in these two treatment groups spawned during the last three weeks of the seven-week feeding experiment. Lipid supplements had a significant influence on hemocyte concentration, phagocytic index and non-stimulated hemocyte ROS production. After 4 weeks, highest hemocyte concentrations were found in oysters fed on a supplement of 50% lipid emulsion compared with those fed on other diets but the hemocytes derived from these oysters had the lowest short-term phagocytic index. After 7 weeks of dietary conditioning, the ROS production in non-stimulated hemocytes of oysters fed 10% and 50% lipid emulsion declined. These results suggested that 20:5n-3, and perhaps its eicosanoid metabolites, affected oyster hemocyte functions; however, the reproductive status of oysters may also have interfered with the 20:5n-3 dietary effect. (c) 2007 Elsevier B.V. All rights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2007/publication-2604.pdf

DOI:10.1016/j.jembe.2006.12.021	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/2604/	
Formato: 	application/pdf	
Fonte: 	Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2007-05 , Vol. 343 , N. 2 , P. 261-275	
Fonte: 	2007 Elsevier B.V. All rights reserved