Data/hora: 01/06/2024 10:58:18

Provedor de dados: | 5
País: | France
Título: | Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph
Autores: | Hubert, Florence; Van Der Knaap, Wil; Noel, Thierry; Roch, Philippe.
Data: | 1996
Ano: | 1996
Palavras-chave: | Cytotoxicité; Antibactérien; Hémolymphe; Lysat d'hémocyte; Moule; Huître; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas; Cytotoxicity; Antibacterial; Hemolymph; Hemocyte lysate; Musssel; Oyster; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas.
Resumo: | Mussel (Mytilus galloprovincialis) plasma contains cytotoxic activity against both vertebrate (erythrocytes and mouse tumour) and protozoan cells. Procaryotes (Escherichia coli and Vibrio alginolyticus) were not sensitive to the cytotoxicity. The activity was still present in dialyzed samples but was inhibited by heating at 45°C. Large individual variability which was not correlated with protein concentration and an increasing number of reactive specimens following injection, suggested that naive mussels were in various stages of immune response. Purification by anion exchange chromatography followed by gel filtration revealed a 320 kDa cytotoxic polymeric protein that acts through a polymerization process after binding onto target cell membranes as revealed by ultrastructural observation. European and Pacific oysters (Ostrea edulis and Crassostrea gigas) expressed antibactenal activity against both Gram negative and Gram positive bacteria which was most probably due to small proteins. When tested against the marine pathogenic Vibrio alginolyticus, hemocyte lysates of both species were more active than cell-free plasma. Antibacterial activity showed significant individual variability that was dramatically reduced by stimulation through mechanical stress or injection. The number of spontaneously active Pacific oysters increased from 50 to 100% following a single injection of bacteria. These results strongly support the view that bivalve molluscs possess sensitive immuno-defense mechanisms that will greatly aid the development of aquaculture systems by employing refined techniques of transgenesis.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/00039/15008/12333.pdf
Editor: | EDP Sciences
Relação: | http://archimer.ifremer.fr/doc/00039/15008/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (EDP Sciences), 1996 , Vol. 9 , P. 115-124
Fonte: | 1996 EDP Sciences