Data/hora: 06/06/2024 18:42:37

Provedor de dados: | 5
País: | France
Título: | Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes
Autores: | Duperron, Sebastien; Fiala Medioni, Aline; Caprais, Jean-claude; Olu, Karine; Sibuet, Myriam.
Data: | 2007
Ano: | 2007
Palavras-chave: | Eastern Mediterranean; Cold seeps; Lucinoma; Lucinidae; Sulphide oxidizing bacteria; Symbiosis.
Resumo: | Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase alpha subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and delta C-13 values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-2176.pdf

DOI:10.1111/j.1574-6941.2006.00194.x

Editor: | Blackwell science
Relação: | http://archimer.ifremer.fr/doc/00000/2176/
Formato: | application/pdf
Fonte: | FEMS Microbiology Ecology (0168-6496) (Blackwell science), 2007 , Vol. 59 , N. 1 , P. 64-70
Fonte: | 2007 Blackwell Publishing, Inc.