Data/hora: 01/06/2024 06:44:59

Provedor de dados: 	5	
País: 	France	
Título: 	Cultivating the uncultured: limits, advances and future challenges	
Autores: 	Alain, Karine; Querellou, Joel.	
Data: 	2009-07	
Ano: 	2009	
Palavras-chave: 	High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.	
Resumo: 	Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we focus on the limiting factors surrounding cultivation. We discuss several cultivation obstacles, including the loss of microbial cell-cell communication following species isolation. Future research directions, including the refinement of culture media, strategies based on cell-cell communication and high-throughput innovations, are reviewed. We further propose that a combination of these approaches is urgently required to promote cultivation of uncultured species, thereby dawning a new era in the field.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2009/publication-6820.pdf

DOI:10.1007/s00792-009-0261-3	
Editor: 	Springer	
Relação: 	http://archimer.ifremer.fr/doc/00000/6820/	
Formato: 	application/pdf	
Fonte: 	Extremophiles (1431-0651) (Springer), 2009-07 , Vol. 13 , N. 4 , P. 583-594	
Fonte: 	2009 Springer.  Part of Springer Science+Business Media