Data/hora: 01/06/2024 11:24:56

Provedor de dados: 	5	
País: 	France	
Título: 	First insight on metabolomics workflows and differences between dinoflagellate: characterization and discrimination of modern and ancient strains and their algal growth	
Autores: 	Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele.	
Data: 	2018-05-23	
Ano: 	2018	
Resumo: 	Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible adaptive responses of phytoplankton to these modifications of coastal ecosystems. Strains of S. donghaienis were obtained from sediments isotopically dated to 1986 (ancient) and 2006 (modern) while those of A. minutum dated from 1996 (ancient) and 2006 (modern). In total 84 samples were obtained, with each experimental condition (species, age, growth phase) being cultured in triplicate in phosphate depleted conditions. More specifically, two workflows have been used for univariate and multivariate modelling on: (i)a chemometrics platform, Agilent Mass Profiler Professional (MPP) (ii) a collaborative portal dedicated to metabolomic data processing, analysis and annotation “Workflow4Metabolomics”	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00440/55199/56664.pdf

https://archimer.ifremer.fr/doc/00440/55199/	
Editor: 	11èmes Journées Scientifiques du Réseau Francophone de Métabolomique et Fluxomique (RMFM). 23-25 mai 2018, Liège, Belgique	
Formato: 	application/pdf	
Fonte: 	info:eu-repo/semantics/openAccess

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