Data/hora: 06/06/2024 15:56:08

Provedor de dados: 	5	
País: 	France	
Título: 	High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas	
Autores: 	Rosa, Rafael D.; Alonso, Pascal; Santini, Adrien; Vergnes, Agnes; Bachere, Evelyne.	
Data: 	2015-04	
Ano: 	2015	
Palavras-chave: 	Mollusk bivalve; Antimicrobial peptide; Polymorphism; Gene copy number variation; High-throughput microfluidic RT-qPCR; Vibrio infection.	
Resumo: 	We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00239/35009/33545.pdf

DOI:10.1016/j.dci.2014.12.002

https://archimer.ifremer.fr/doc/00239/35009/	
Editor: 	Elsevier	
Formato: 	application/pdf	
Fonte: 	Developmental &amp; Comparative Immunology (0145-305X) (Elsevier), 2015-04 , Vol. 49 , N. 2 , P. 231-238	
Fonte: 	2014 Elsevier Ltd. All rights reserved.

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