Data/hora: 06/06/2024 13:44:32

Provedor de dados: | 5
País: | France
Título: | The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta
Autores: | Henneke, Ghislaine; Raffin, Jean-paul; Ferrari, Elena; Jonsson, Zophonías; Dietrich, Jacques; Hubscher, Ulrich.
Data: | 2000-09
Ano: | 2000
Palavras-chave: | DNA polymerase delta; Conservation; Thermococcus fumicolans; Archaea; Hyperthermophile; PCNA.
Resumo: | We have cloned the gene encoding proliferating cell nuclear antigen (PCNA) from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M-r of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNA were required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta. Our data suggest that PCNA has been functionally conserved not only within eukaryotes but also from hyperthermophilic euryarchaeotes to mammals.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2000/publication-659.pdf

DOI:10.1006/bbrc.2000.3481

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/659/
Formato: | application/pdf
Fonte: | Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2000-09 , Vol. 276 , N. 2 , P. 600-606
Fonte: | 2000 Academic Press. All rights reserved