Data/hora: 06/06/2024 03:49:37

Provedor de dados: | 5
País: | France
Título: | Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)
Autores: | Lazerges, M; Perrot, H; Antoine, Elisabeth; Defontaine, Audrey; Compere, Chantal.
Data: | 2006
Ano: | 2006
Palavras-chave: | DNA; Alexandrium minutum; Microbalance.
Resumo: | We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2006/publication-1354.pdf

DOI:10.1016/j.bios.2005.05.013

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/1354/
Formato: | application/pdf
Fonte: | Biosensors and Bioelectronics (0956-5663) (Elsevier), 2006 , Vol. 21 , N. 7 , P. 1355-1358
Fonte: | 2005 Elsevier B.V. All rights reserved