Data/hora: 10/06/2024 05:45:33

Provedor de dados: 	5	
País: 	France	
Título: 	Métabolisme énergétique du spermatozoïde d'huître creuse (Crassostrea gigas)

Energy metabolism of Pacific oyster (Crassostrea gigas) spermatozoa	
Autores: 	Boulais, Myrina.	
Data: 	2014-12-11	
Ano: 	2014	
Palavras-chave: 	Fécondation; Sperme; Analyse du mouvement; Métabolisme énergétique; RMN; Cytométrie en flux; Crassostrea gigas; Reproduction; Fertilization; Sperm; Movement analysis; Energetic metabolism; NMR; Flow cytometry.	
Resumo: 	The present study aims at describing the energy metabolism of Pacific oyster, Crassostrea gigas, spermatozoon. The main objectives are: (1) describing the kinetic of cellular and biochemical characteristics of Pacific oyster spermatozoon during its movement phase, (2) identifying the metabolic pathways to produce energy and their substrates, (3) determining the involvement of energy metabolism in fertilization success. The results showed the long duration (ranging 24 to 72h) of the movement of Pacific oyster spermatozoon and the maintenance of a high intracellular ATP content until the end of the movement phase. The role of oxidative phosphorylation (OXPHOS) in ATP production was highlighted. Alternative ATP producing pathways might contribute to sustain energy production when OXPHOS is impaired, showing the high adaptation capacity of Pacific oyster spermatozoa. An increase of phosphoarginine content observed at the end of the movement phase suggests energy storage capacity. Endogenous substrates involved in ATP synthesis must be determined. Both the high regulation capacity of the energy metabolism of Pacific oyster spermatozoa and their ability to restore energy stores catabolized during movement likely contribute to the successful settlement observed in the wild. In conclusion, this work provided a fine description of energetic metabolism of Pacific oyster spermatozoon.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	https://archimer.ifremer.fr/doc/00498/60940/64334.pdf

https://archimer.ifremer.fr/doc/00498/60940/	
Editor: 	Université de Bretagne occidentale	
Formato: 	application/pdf	
Fonte: 	info:eu-repo/semantics/openAccess

restricted use