Data/hora: 02/06/2024 15:45:55

Provedor de dados: 	5	
País: 	France	
Título: 	Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout	
Autores: 	Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome.	
Data: 	2014-12	
Ano: 	2014	
Palavras-chave: 	PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity.	
Resumo: 	A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic extract from a PAH-contaminated sediment (Seine estuary, France) and two petrogenic extracts from Arabian Light and Erika oils, at two environmental concentrations, 3 and 10 μg g−1 sum of PAHs. The degree and spectrum of toxicity were different according to the extract considered. Acute effects including embryo mortality and decreased hatching success were observed only for Erika oil extract. Arabian Light and pyrolytic extracts induced mainly sublethal effects including reduced larvae size and hemorrhages. Arabian Light and Erika extracts both induced repairable DNA damage as revealed by the comet assay versus the micronucleus assay. The concentration and proportion of methylphenanthrenes and methylanthracenes appeared to drive the toxicity of the three PAH fractions tested, featuring a toxic gradient as follows: pyrolytic &lt; Arabian Light &lt; Erika. The minimal concentration causing developmental defects was as low as 0.7 μg g−1 sum of PAHs, indicating the high sensitivity of the assay and validating its use for toxicity assessment of particle-bound pollutants.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00188/29919/28384.pdf

DOI:10.1007/s11356-014-2804-0

http://archimer.ifremer.fr/doc/00188/29919/	
Editor: 	Springer Heidelberg	
Formato: 	application/pdf	
Fonte: 	Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2014-12 , Vol. 21 , N. 24 , P. 13720-13731	
Fonte: 	Springer-Verlag Berlin Heidelberg 2014

info:eu-repo/semantics/openAccess

restricted use