Data/hora: 01/06/2024 08:14:38

Provedor de dados: | 5
País: | France
Título: | Rapid detection and quantification of the marine toxic algae, Alexandrium minutum, using a super-paramagnetic immunochromatographic strip test
Autores: | Gas, Fabienne; Baus, Beatrice; Quere, Julien; Chapelle, Annie; Dreanno, Catherine.
Data: | 2016-01
Ano: | 2016
Palavras-chave: | Alexandrium minutum; Detection; Quantification; Dipstick lateral flow immunoassay; Super-paramagnetic.
Resumo: | The dinoflagellates of Alexandrium genus are known to be producers of paralytic shellfish toxins that regularly impact the shellfish aquaculture industry and fisheries. Accurate detection of Alexandrium including A. minutum is crucial for environmental monitoring and sanitary issues. In this study, we firstly developed a quantitative lateral flow immunoassay (LFIA) using super-paramagnetic nanobeads for A. minutum whole cells. This dipstick assay relies on two distinct monoclonal antibodies used in a sandwich format and directed against surface antigens of this organism. No sample preparation is required. Either frozen or live cells can be detected and quantified. The specificity and sensitivity are assessed by using phytoplankton culture and field samples spiked with a known amount of cultured A. minutum cells. This LFIA is shown to be highly specific for A. minutum and able to detect reproducibly 105 cells/L within 30 min. The test is applied to environmental samples already characterized by light microscopy counting. No significant difference is observed between the cell densities obtained by these two methods. This handy super-paramagnetic lateral flow immnunoassay biosensor can greatly assist water quality monitoring programs as well as ecological research.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00286/39695/38152.pdf

DOI:10.1016/j.talanta.2015.10.036

http://archimer.ifremer.fr/doc/00286/39695/

Editor: | Elsevier Science Bv
Formato: | application/pdf
Fonte: | Talanta (0039-9140) (Elsevier Science Bv), 2016-01 , Vol. 147 , P. 581-589

Fonte:

2015 Elsevier B.V. All rights reserved.

info:eu-repo/semantics/openAccess

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